Self organization of the actomyosin cytoskeleton in fibroblasts

The actomyosin network is essential for cellular functions such as migration and adhesion, where force generation and sensing are important properties. Parallel actomyosin stress fibers crosslinked by a-actinin can additionally be crosslinked by highly structured arrays of myosin, called myosin stacks, that form when myosin regions in neighboring stress fibers align with each other [Hu et al., 2017].
In a recent study we could additionally show that this specific order of the cytoskeleton plays a role in the capacity of cells to generate forces [Hu, Grobe et al., 2019]. However, the mechanism underlying the ordering of the cytoskeleton in 2D and 3D, how myosin stacks are maintained within a cell as well as the role myosin stacks play in physiological and pathophysiological functions remain poorly understood and is the current focus of this study.